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Cells were resuspended in NP-40 lysis buffer (40 mM HEPES (pH 7.5), 200 mM NaCl, 5 mM b-glycerophosphate, 0.1 mM sodium orthovanadate, Roche EDTA free complete protease inhibitor, 2 mM TCEP, 0.4% NP-40) and sonicated in a Bioruptor sonication system from Diagenode Inc

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Some of its mid-size retail spaces have never had a tenant in the six years it has been open