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Astrocytes can contribute in a disease-stage-dependent manner by chemokines (CCL2 and CXCL10), VEGF, and MMP-2/9, which facilitate leukocyte recruitment and blood–brain barrier disruption during active lesions [184]

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In adults IM sites include the deltoid, ventrogluteal, vastus lateralis and dorsal gluteal (with careful land marking to avoid the sciatic nerve.) In children, you do not inject into the gluteal muscle until they have reached approximately the age of 2 and have been walking for a time sufficient to develop the muscle.