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You’ll wash the column to get rid of as many contaminating proteins as possible, then elute the tagged proteins by adding a buffer component that interferes with the ligand-tag interaction (Figure 6)

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[DOI] [Google Scholar] 547.Lv X., Wu Y., Gong M., Deng J., Gu Y., Liu Y., Li J., Du G., Ledesma-Amaro R., Liu L., et al