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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 µg ml –1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml –1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)