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Michaelis–Menten kinetics for Fe III reduction were measured at room temperature in a final volume of 0.5 ml with 50 mM Tris pH 7.0 and 250 mM NaCl with 800 µM ferrozine, supplemented with 0.5 µM SpNOX, 1 µM FAD and varying Fe III -EDTA concentrations

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Front Endocrinol (Lausanne) 2021;12:727272