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Vitamin D has demonstrated neuroprotective effects in PD models by reducing dopaminergic neuron apoptosis and suppressing inflammatory signaling [88], as well as enhancing striatal dopamine release and nigral dopamine content [89]

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To further investigate microbial differences between the EMs and control groups, multiple statistical and machine learning methods were employed—including EdgR, DESeq2, zero-inflated negative binomial model (ZINB), negative binomial model (NEGBIN), compound Poisson lognormal model (CPLM), Random Forest, and linear discriminant analysis (LDA) effect size (LEfSe)