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It is hypothesized that the inhibitory effects of factors such as oocyte maturation inhibitor or luteinization inhibitor are overcome at ovulation (30)

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A total of 60 μg of protein was subjected to electrophoresis (Bio-Rad), transferred to 2.2 μm polyvinylidene fluoride membranes (1620177, Bio-Rad) and probed with primary antibodies followed by HRP-conjugated Anti-Rabbit IgG (H + L) antibody (ab6721, Abcam) or HRP-Conjugated Anti-Mouse IgG (H + L) antibody (ab6789, Abcam)

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