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10.1186/s10020-025-01147-z 72 PintoM.MehbodA

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For four of these metabolites (homotaurine, N -acetyl-phenylalanylleucine/isoleucine, 3-hydroxypropane-1-sulfonic acid, and N 1 -methyl-imidazolelactic acid), the chemical standard did not match to the original tissue peak, but instead matched a peak with a distinct MS/MS spectrum and/or a retention time difference that could not be explained by chromatographic drift (see also ‘Success rate of metabolite discovery’)