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Along with the presence of high- and low-abundant microbes, the maintenance of the intestinal epithelial barrier is further maintained by critical players, including immune cells (mononuclear macrophages, T-cells, and innate lymphoid cells), enteric glial cells (EGCs), and the intrinsic/extrinsic enteric-associated-neurons (iEANs/eEANs) of the ENS (Gabanyi et al., 2016