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Among inflammatory cytokines, IL-1β (Interleukin-1β) is one of the most extensively studied in OA.Exposure of chondrocytes to IL‑1β results in pronounced up‑regulation of matrix metalloproteinases—particularly MMP‑1, MMP‑3, and MMP‑13—which catalyze the degradation of collagen and proteoglycans within the cartilage extracellular matrix, ultimately compromising its structural integrity

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Any leftover solution should be thrown away after use