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In terms of mechanisms of action, 6G induced autophagy by significantly increasing the protein expression levels of LC3B-II and Beclin-1, significantly decreasing the protein expression levels of GPX4 and Nrf2 and increasing the ROS level in prostate cancer cells (119)

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However, this Cys-targeted proteomics analysis should be complemented by the enzymatic activity measurement of an individual target protein and immunoprecipitation followed by immunoblot analysis with S-NO-Cys, glutathione, or 3-nitro-tyrosine