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Recombinant human active caspase-3, human WT, C45A, C180A, C371A His-sumo-GSDME-eGFP were from BioGot (Nanjing, China), recombinant human His-GSDME (CSB-EP006766HU) was from CUSABIO (Wuhan, China), recombinant human active caspase-4 was purchased from Biovision (1084-100, San Francisco, USA), recombinant human His-sumo-GSDMD-eGFP, His-sumo-GSDME were from Detai Bio (Nanjing, China), recombinant human TNFα (300-01 A) was purchased from Peprotech (Cranbury, USA), recombinant perforin/PFR (APB317Hu02) was purchased from Cloud-Clone (Wuhan, China), recombinant granzyme B/GZMB (HY-P75157) was purchased from MCE (NJ, USA)