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FIGURE 1 3.2 Cell transformation assay The effect of DEHP on the transformation frequency of BALB/c 3T3 A31-1-1 cells was assessed according to the protocol recommended by ECVAM ( The positive control MCA (4 μg/mL) induced a statistically significant increase in the number of transformed type III foci, which were almost absent in untreated and solvent-treated cells (Figure 2)