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Relevant fractions were concentrated and then loaded on a gel filtration column, which was equilibrated by size-exclusion chromatography (SEC) buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl 2 , and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP))

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Folia Med Cracov (2018) 58:119–30

it is essential to combine its use with regular exercise for optimal results