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The PCR mixtures contained 1 μl of cDNA synthesized from 2 μg of total RNA, 3.2 μl of dNTP, 2 μl of 10 × ExTaq buffer, 0.1 μl of ExTaq DNA polymerase (Takara, Shiga, Japan), and 20 pmol each of sense and antisense primers from UGT1A, UGT2B, GLYAT, and beta-actin in a total volume of 20 μl