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Mechanism of thymidylate synthase inhibition by methotrexate in human neoplastic cell lines and normal human myeloid progenitor cells
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In intestinal HT-29/B6 cells, TNF-α treatment resulted in decreased TJ strand number and complexity and increased frequency of strand breaks.66 TNF-α also inhibits occludin promoter activity43 and causes redistribution of occludin, ZO-1, and claudin-1.67 In vitro and in vivo , transcription and translation of MLCK, the major effector responsible for TNF-α-induced TJ modulation, are increased by TNF-α.43,67,68 Other pro-inflammatory cytokines may also mediate barrier function through modulation of MLCK activity
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