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Blocking NNMT is therefore proposed to raise intracellular SAM, shift nicotinamide back toward NAD+ production, and alter the epigenetic landscape of adipocytes in ways that reduce lipid storage

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Bisulfite sequencing PCR To assess the impact of VCD and supplemental vitamin B12 on the methylation levels of 17 CpG sites located in the promoter region and proximal portion of the first exon of CCL11 , DNA libraries obtained from cultures of endpoints A and B were analyzed using Bisulfite Sequencing PCR (BSP)