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Preclinical studies indicate that systemic glutamine deprivation may induce muscle atrophy and intestinal mucosal damage (128)
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1 ml of 50 mM potassium phosphate buffer (pH 7.0), containing 10% (w/v) polyvinylpyrrolidone (PVP), 0.25% (v/v) Triton X-100, 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 mM ASA were added in a homogenate of 100 mg (FW) of leaf tissues to measure glutathione reductase (GR, EC 1.6.4.2), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and monodehydroascorbate reductase (MDHAR, EC 1.6.5.4)