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The plate was then incubated at 37 °C for 45 minutes in a shaker water bath then cooled and the absorbance of the pink colored chromophore was measured at 540 nm using ELISA reader, against a blank treated in a similar manner to the test but using 100µl distilled water instead of the sample
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Integrated-care models staffed by movement-disorder specialists, physiotherapists, psychologists, and nutritionists not only improve motor scales but also trim emergency-department visits and caregiver burden, underscoring the economic imperative of multidisciplinarity [327]
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